mouse mab cp-13 antibody Search Results


primary antibody against cp13  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc primary antibody against cp13
A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of <t>CP13,</t> PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).
Primary Antibody Against Cp13, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/Phospho-Tau+(Ser202)+Rabbit+mAb/pmc09481623-145-3-11
Average 93 stars, based on 1 article reviews
primary antibody against cp13 - by Bioz Stars, 2026-09
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synucleinopathies classification21 25 32  (Proteintech)
94
Proteintech synucleinopathies classification21 25 32
A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of <t>CP13,</t> PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).
Synucleinopathies Classification21 25 32, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/TDP-43+Antibody/pm35481808-62-34-57
Average 94 stars, based on 1 article reviews
synucleinopathies classification21 25 32 - by Bioz Stars, 2026-09
94/100 stars
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phospho tau cp13  (Feinstein Institute)
86
Feinstein Institute phospho tau cp13
A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of <t>CP13,</t> PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).
Phospho Tau Cp13, supplied by Feinstein Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/anti+cp13+phospho+tau/pmc10922743-53-19-31
Average 86 stars, based on 1 article reviews
phospho tau cp13 - by Bioz Stars, 2026-09
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tau  (Proteintech)
95
Proteintech tau
A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of <t>CP13,</t> PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).
Tau, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/TAU+Antibody/pmc03136530-41-15-37
Average 95 stars, based on 1 article reviews
tau - by Bioz Stars, 2026-09
95/100 stars
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4r tau specific antibody rd4  (Feinstein Institute)
86
Feinstein Institute 4r tau specific antibody rd4
A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of <t>CP13,</t> PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).
4r Tau Specific Antibody Rd4, supplied by Feinstein Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/4r+antibody+rd4+specific+tau/pmc11449633-51-7-26
Average 86 stars, based on 1 article reviews
4r tau specific antibody rd4 - by Bioz Stars, 2026-09
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deep space black hrp vector red alkaline phosphatase ap substrate kit ppn cp 13  (Vector Laboratories)
95
Vector Laboratories deep space black hrp vector red alkaline phosphatase ap substrate kit ppn cp 13
Immunohistochemistry Information for Each Nucleus
Deep Space Black Hrp Vector Red Alkaline Phosphatase Ap Substrate Kit Ppn Cp 13, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/VECTOR+Black+Alkaline+Phosphatase+(AP)+Substrate+Kit/pmc06251636-131-178-197
Average 95 stars, based on 1 article reviews
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antiytau cp 13  (Proteintech)
93
Proteintech antiytau cp 13
Immunohistochemistry Information for Each Nucleus
Antiytau Cp 13, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/PRSS8+Antibody/10__1097_slash_nen__0b013e31822c62cf-41-17-44
Average 93 stars, based on 1 article reviews
antiytau cp 13 - by Bioz Stars, 2026-09
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vectastain abc ap kit  (Vector Laboratories)
96
Vector Laboratories vectastain abc ap kit
Immunohistochemistry Information for Each Nucleus
Vectastain Abc Ap Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/VECTASTAIN+ABC-AP+Staining+KIT+(Alkaline+Phosphatase%2C+Standard)/pmc06251636-186-87-93
Average 96 stars, based on 1 article reviews
vectastain abc ap kit - by Bioz Stars, 2026-09
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vector red alkaline phosphatase ap substrate kit  (Vector Laboratories)
96
Vector Laboratories vector red alkaline phosphatase ap substrate kit
Immunohistochemistry Information for Each Nucleus
Vector Red Alkaline Phosphatase Ap Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc06251636-186-102-110
Average 96 stars, based on 1 article reviews
vector red alkaline phosphatase ap substrate kit - by Bioz Stars, 2026-09
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biotinylated horse antimouse  (Vector Laboratories)
96
Vector Laboratories biotinylated horse antimouse
Immunohistochemistry Information for Each Nucleus
Biotinylated Horse Antimouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/Biotinylated+Horse+Anti-Mouse+IgG+Antibody/pmc06251636-131-194-197
Average 96 stars, based on 1 article reviews
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goatamouse irdye 680lt  (LI-COR)
99
LI-COR goatamouse irdye 680lt
Immunohistochemistry Information for Each Nucleus
Goatamouse Irdye 680lt, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/GOATaMOUSE+IRDye+680LT/custom%40926-68020%4028972160
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cyp1a1/1a2 antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cyp1a1/1a2 antibody
Immunohistochemistry Information for Each Nucleus
Cyp1a1/1a2 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+mab+cp-13+antibody/CYP1A1%2F1A2+Antibody/custom%40v7709%4035508385
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Image Search Results


A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of CP13, PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).

Journal: Translational Psychiatry

Article Title: Identification of a pleiotropic effect of ADIPOQ on cardiac dysfunction and Alzheimer’s disease based on genetic evidence and health care records

doi: 10.1038/s41398-022-02144-0

Figure Lengend Snippet: A–D Validation of the functional impact of the 3 identified AD/D variants. We constructed site-directed mutant Neuro-2a cell lines ( MTHFD1L -M, DPP10 -M, and ADIPOQ -M). We assessed the abundance of each cell line of CP12, PHF1, and tau proteins via three biological replicates. A Loading abundance of CP13, PHF1, and tau proteins, as well as β-actin in neuronal cell lines by transfected genes. ‘M’ = mutated. ‘W’ = wild type. B – D Levels of aggregation of CP13, PHF1, and tau normalized by β-actin. ADIPOQ -M displayed abnormal aggregation of tau and CP13 (D) ( p < 0.05, t -test). E Analysis of an enriched pathway of 473 selected differentially expressed genes (DEGs) (FDR p < 0.05, log 2 fold change >5) in ADIPOQ knockout cells, H9c2 (rat cardiac cell). Cardiac dysfunction and cognition impairment pathways were enriched in 473 DEGs (FDR p < 0.05, hypergeometric test). F–H Functional impact of the ADIPOQ variant on a population scale using the UK Biobank. We selected 69 individuals from the minor allele group of ADIPOQ (c.268G>A) and 276 from the major allele group based on the propensity score matching analysis. F The average heart wall thickness across 16 sites was significantly increased in the ADIPOQ -M group ( ADIPOQ c.268A) ( p = 0.0023, Wilcoxon test). G Differences in mean reaction time (RT), calculated over 12 rounds, to press a “snap” button when both cards presented matched correctly. The X -axis represents the difference in the measured RTs between the initial assessment (0 years) and the third assessment (5–10 years later). Each plot shows the RTs by allele group ( ADIPOQ -W and ADIPOQ -M). H Difference in the mean RTs between the baseline and the third assessment. The minor allele group ( ADIPOQ -M) showed a longer mean RT than the major allele group in the third assessment compared to the first assessment ( p < 0.05, t-test).

Article Snippet: We used specific primary antibody against CP13 (Anti-CP13, phospho-tau ser202) (39357, Cell Signaling Technology).

Techniques: Biomarker Discovery, Functional Assay, Construct, Mutagenesis, Transfection, Knock-Out, Variant Assay

Immunohistochemistry Information for Each Nucleus

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Selective Vulnerability of Brainstem Nuclei in Distinct Tauopathies: A Postmortem Study

doi: 10.1093/jnen/nlx113

Figure Lengend Snippet: Immunohistochemistry Information for Each Nucleus

Article Snippet: Positive and negative controls were included in all IHC runs. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Region of Interest First Primary Antibody and Dilution Detection of First Primary Antibody Second Primary Antibody and Dilution Detection of Second Primary Antibody Chromogen for Detecting First Primary Antibody Chromogen for Detecting Second Primary Antibody DRN CP-13, mouse (p-tau, serine 202)1: 900, gift of Peter Davies, NY Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tryptophan hydroxylase (PH8, MAB5278 mouse, 1: 900, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP (BR14015, Biocare Medical) Vector Red Alkaline Phosphatase AP Substrate kit (SK-5100, Vector Labs) GCN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) GAD1 (MAB5406, rabbit, 1: 300, Millipore) Mach 4 MR AP polymer (MRAP 536G, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit LC CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit PPN CP-13, mouse, 1: 700 Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC HRP kit (Vector Labs AK-5000) glutamate receptor (VGlut2, MAB 5504, mouse, 1: 800, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit SN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit Open in a separate window DRN, dorsal raphe nucleus; GCN, gigantocellular nucleus; HRP, horseradish peroxidase; AP, alkaline phosphatase; LC, locus coeruleus; PPN, pedunculopontine nucleus; SN, substantia nigra; p-tau, phosphorylated tau.

Techniques: Immunohistochemistry, Plasmid Preparation

Histological section (8-µm-thick) through the (A) dorsal raphe nucleus (double immunostained for phospho-tau [CP-13, in brown] and tryptophan hydroxylase [PH8, in red]) and (B) substantia nigra (CP-13, in brown) and tyrosine hydroxylase (PH8, in red)). The figure depicts some examples on how the neurons were classified: (1 and 6) tau-positive/NEU-positive colocalized; (2 and 5) neuron positive for neurotransmitter only (NEU-positive); (3) positive for phospho-tau only (tau-positive); and (4) neuron negative for phospho-tau and not expressing the neurotransmitter of interest.

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Selective Vulnerability of Brainstem Nuclei in Distinct Tauopathies: A Postmortem Study

doi: 10.1093/jnen/nlx113

Figure Lengend Snippet: Histological section (8-µm-thick) through the (A) dorsal raphe nucleus (double immunostained for phospho-tau [CP-13, in brown] and tryptophan hydroxylase [PH8, in red]) and (B) substantia nigra (CP-13, in brown) and tyrosine hydroxylase (PH8, in red)). The figure depicts some examples on how the neurons were classified: (1 and 6) tau-positive/NEU-positive colocalized; (2 and 5) neuron positive for neurotransmitter only (NEU-positive); (3) positive for phospho-tau only (tau-positive); and (4) neuron negative for phospho-tau and not expressing the neurotransmitter of interest.

Article Snippet: Positive and negative controls were included in all IHC runs. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Region of Interest First Primary Antibody and Dilution Detection of First Primary Antibody Second Primary Antibody and Dilution Detection of Second Primary Antibody Chromogen for Detecting First Primary Antibody Chromogen for Detecting Second Primary Antibody DRN CP-13, mouse (p-tau, serine 202)1: 900, gift of Peter Davies, NY Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tryptophan hydroxylase (PH8, MAB5278 mouse, 1: 900, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP (BR14015, Biocare Medical) Vector Red Alkaline Phosphatase AP Substrate kit (SK-5100, Vector Labs) GCN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) GAD1 (MAB5406, rabbit, 1: 300, Millipore) Mach 4 MR AP polymer (MRAP 536G, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit LC CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit PPN CP-13, mouse, 1: 700 Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC HRP kit (Vector Labs AK-5000) glutamate receptor (VGlut2, MAB 5504, mouse, 1: 800, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit SN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit Open in a separate window DRN, dorsal raphe nucleus; GCN, gigantocellular nucleus; HRP, horseradish peroxidase; AP, alkaline phosphatase; LC, locus coeruleus; PPN, pedunculopontine nucleus; SN, substantia nigra; p-tau, phosphorylated tau.

Techniques: Expressing

Immunohistochemistry Information for Each Nucleus

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Selective Vulnerability of Brainstem Nuclei in Distinct Tauopathies: A Postmortem Study

doi: 10.1093/jnen/nlx113

Figure Lengend Snippet: Immunohistochemistry Information for Each Nucleus

Article Snippet: Positive and negative controls were included in all IHC runs. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Region of Interest First Primary Antibody and Dilution Detection of First Primary Antibody Second Primary Antibody and Dilution Detection of Second Primary Antibody Chromogen for Detecting First Primary Antibody Chromogen for Detecting Second Primary Antibody DRN CP-13, mouse (p-tau, serine 202)1: 900, gift of Peter Davies, NY Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tryptophan hydroxylase (PH8, MAB5278 mouse, 1: 900, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP (BR14015, Biocare Medical) Vector Red Alkaline Phosphatase AP Substrate kit (SK-5100, Vector Labs) GCN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) GAD1 (MAB5406, rabbit, 1: 300, Millipore) Mach 4 MR AP polymer (MRAP 536G, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit LC CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit PPN CP-13, mouse, 1: 700 Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC HRP kit (Vector Labs AK-5000) glutamate receptor (VGlut2, MAB 5504, mouse, 1: 800, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit SN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit Open in a separate window DRN, dorsal raphe nucleus; GCN, gigantocellular nucleus; HRP, horseradish peroxidase; AP, alkaline phosphatase; LC, locus coeruleus; PPN, pedunculopontine nucleus; SN, substantia nigra; p-tau, phosphorylated tau.

Techniques: Immunohistochemistry, Plasmid Preparation

Immunohistochemistry Information for Each Nucleus

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Selective Vulnerability of Brainstem Nuclei in Distinct Tauopathies: A Postmortem Study

doi: 10.1093/jnen/nlx113

Figure Lengend Snippet: Immunohistochemistry Information for Each Nucleus

Article Snippet: Positive and negative controls were included in all IHC runs. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Region of Interest First Primary Antibody and Dilution Detection of First Primary Antibody Second Primary Antibody and Dilution Detection of Second Primary Antibody Chromogen for Detecting First Primary Antibody Chromogen for Detecting Second Primary Antibody DRN CP-13, mouse (p-tau, serine 202)1: 900, gift of Peter Davies, NY Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tryptophan hydroxylase (PH8, MAB5278 mouse, 1: 900, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP (BR14015, Biocare Medical) Vector Red Alkaline Phosphatase AP Substrate kit (SK-5100, Vector Labs) GCN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) GAD1 (MAB5406, rabbit, 1: 300, Millipore) Mach 4 MR AP polymer (MRAP 536G, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit LC CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit PPN CP-13, mouse, 1: 700 Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC HRP kit (Vector Labs AK-5000) glutamate receptor (VGlut2, MAB 5504, mouse, 1: 800, Millipore) Biotinylated Horse Antimouse (Vector Labs BA-2000) + Vectastain ABC AP kit (AK-5000, Vector Labs) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit SN CP-13, mouse, 1: 900 Mach 2 Mouse HRP (MHRP520G, Biocare Medical) Tyrosine hydroxylase (TH, PA-1-4605, rabbit, 1: 700, Fisher Scientific) Mach 2 Rabbit AP (RALP525, Biocare Medical) Deep Space Black HRP Vector Red Alkaline Phosphatase AP Substrate kit Open in a separate window DRN, dorsal raphe nucleus; GCN, gigantocellular nucleus; HRP, horseradish peroxidase; AP, alkaline phosphatase; LC, locus coeruleus; PPN, pedunculopontine nucleus; SN, substantia nigra; p-tau, phosphorylated tau.

Techniques: Immunohistochemistry, Plasmid Preparation